Quantifying single-cell diacylglycerol signaling kinetics after uncaging.

ABSTRACT: Studying the role of molecularly distinct lipid species in cell signaling remains challenging due to a scarcity of methods for performing quantitative lipid biochemistry in living cells. We have recently used lipid uncaging to quantify lipid-protein affinities and rates of lipid trans-bilayer movement and turnover in the diacylglycerol signaling pathway. This approach is based on acquiring live-cell dose-response curves requiring light dose titrations and experimental determination of uncaging photoreaction efficiency. We here aimed to develop a methodological approach that allows us to retrieve quantitative kinetic data from uncaging experiments that 1) require only typically available datasets without the need for specialized additional constraints and 2) should in principle be applicabl

SUBMITTER: Gonzales DT 

PROVIDER: S-EPMC10754688 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature