Identification of a suitable method of inoculation for reducing background effect in mock-inoculated controls during gene expression studies in <i>Arabidopsis thaliana</i>.

ABSTRACT: The recent advancement in the field of transcriptome and methylome sequencing helped scientists to analyse the gene expression and epigenetic status of different genes. Several genes and their regulatory pathways have been discovered due to research into plant-microbe interactions. Previous research on plant-Agrobacterium interactions found that the method of inoculation (wounding using a syringe), resulted in altered DNA methylation of the host DNA repair gene promoters. The expression study of host defence genes revealed that the method of inoculation masked the host response to bacteria. It could be possible that these method-induced changes could interfere with various defence regulatory pathways, which otherwise would not be triggered by the bacteria alone. Hence, it would be c

SUBMITTER: Yamuna KT 

PROVIDER: S-EPMC10754790 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature