DTrmt10A impacts Hsp70 chaperone m⁶A levels and the stress response in the Drosophila brain.

ABSTRACT: Chronic cellular stress has a profound impact on the brain, leading to degeneration and accelerated aging. Recent work has revealed the vital role of RNA modifications, and the proteins responsible for regulating them, in the stress response. In our study, we defined the role of CG14618/dTrmt10A, the Drosophila counterpart of human TRMT10A a N¹-methylguanosine methyltransferase, on m⁶A regulation and heat stress resilience in the Drosophila brain. By m⁶A-IP RNA sequencing on Drosophila head tissue, we demonstrated that manipulating dTrmt10A levels indirectly regulates m⁶A levels on polyA + RNA. dTrmt10A exerted its influence on m⁶A levels on transcripts enriched for neuronal signaling and heat stress pathways, similar to the m⁶

SUBMITTER: Perlegos AE 

PROVIDER: S-EPMC10754819 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature