Pseudorabies virus causes splenic injury via inducing oxidative stress and apoptosis related factors in mice.

ABSTRACT: Pseudorabies virus (PRV) is an immunosuppressive virus that causes significant damage to the pig industry. This study aimed to investigate the effects of PRV on oxidative stress and apoptotic related in the spleen of mice to provide basis knowledge for further research on the pathogenesis of PRV in mice model. 36 mice were randomly two groups, the control group which only received 200 μL PBS and infection group which was subcutaneously infected with 200 μL of 1 × 10³ TCID₅₀/100 μL PRV, respectively. Spleen tissues in each group were collected for further experiments at 48, 72, and 96 h post-infection (hpi). Pathological observation was performed by hematoxylin and eosin Y staining. Biochemical and Flow cytometry methods were used to determine the reactive oxygen speci

SUBMITTER: Sun W 

PROVIDER: S-EPMC10754911 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature