Localizing Hormone Receptor Expression to Cellular Compartments in Idiopathic Subglottic Stenosis.

ABSTRACT:

Objectives

Idiopathic subglottic stenosis (iSGS) is an unexplained progressive fibrosis of the upper airway. iSGS almost exclusively affects women; as a result, female hormones (estrogen and progesterone) have been proposed to participate in the pathogenesis of iSGS. Our aim was to localize cell-specific gene expression of estrogen receptors (ESR1 and ESR2) and progesterone receptor (PGR) using an established iSGS single-cell RNA sequencing (scRNAseq) cell atlas.

Study design

Ex vivo molecular study of airway scar and healthy mucosa from iSGS patients.

Methods

An established scRNAseq atlas consisting of 25,974 individually sequenced cells from subglottic scar (n = 7) or matched unaffected mucosa (n = 3) in iSGS patients was interrogated for RNA expression of ESR1, ESR

SUBMITTER: Talatala ERR 

PROVIDER: S-EPMC10755061 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature