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Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation.

ABSTRACT: Apolipoprotein E is a secreted protein involved in mediating lipid distribution and metabolism among cells of specific tissues. The dysregulation of Apolipoprotein E can disturb cholesterol homeostasis, resulting in several diseases, including cardiovascular disease and Alzheimer's disease. The therapeutic potential of Apolipoprotein E against these diseases demonstrates the importance of providing high-quality antibodies for this protein to the scientific community. In this study, we characterized fourteen Apolipoprotein E commercial antibodies for Western Blot and immunoprecipitation, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.

SUBMITTER: Ayoubi R

PROVIDER: S-EPMC10755264 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation.

Ayoubi Riham R Southern Kathleen K Laflamme Carl C

F1000Research 20230929

Apolipoprotein E is a secreted protein involved in mediating lipid distribution and metabolism among cells of specific tissues. The dysregulation of Apolipoprotein E can disturb cholesterol homeostasis, resulting in several diseases, including cardiovascular disease and Alzheimer's disease. The therapeutic potential of Apolipoprotein E against these diseases demonstrates the importance of providing high-quality antibodies for this protein to the scientific community. In this study, we characteri ...[more]

PMID: 38161428

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