Exosomes from adipose-derived stem cells restore fibroblast function and accelerate diabetic wound healing.

ABSTRACT:

Background

Diabetes is common yet challenging chronic disease, that affects a wide range of people around the world. Complex cellular environments around diabetic wounds tend to damage the function of effector cells, including vascular endothelial cells (VECs), fibroblasts and epithelial cells. This study aims to analyze the differences between diabetic wounds and normal skin as well as whether adipose-derived stem cell (ADSC) exosome could promote healing of diabetic wound.

Methods

Human diabetic wounds and normal skin were collected and stained with HE, Masson, CD31 and 8-hydroxy-2 deoxyguanosine immunohistochemical staining. RNA-seq data were collected for further bioinformatics analysis. ADSC exosomes were isolated and identified by transmission electron microscopy (TEM)

SUBMITTER: Yang C 

PROVIDER: S-EPMC10755272 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature