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Brain tissue haemoglobin expression in saline-perfused vs non-perfused rodents.

ABSTRACT: Haemoglobin beta (Hbb) and delta-aminolevulinate synthase 2 (Alas2) messenger RNA (mRNA) is mainly found in immature red blood cells, reticulocytes, and not in mature erythrocytes. However, these are also expressed in other tissues such as brain cells, mostly neurons. Therefore, exact quantification of neural tissue homogenates may be confounded by remaining blood in the brain vasculature that may give falsely high values of Hbb/Alas2 expression. To investigate and compare the contribution of local Hbb/Alas2 expression, we investigated mRNA expression locally in the hippocampus and prefrontal cortex, in post-sacrifice saline-perfused and non-perfused mice and rats. Although there was a higher level of Hbb/Alas2 transcripts in the non-perfused animals, there was a significant mRNA expression in perfused brains that could at most partially be explained by remaining blood. Finally, we suggest that saline-perfusion should be recommended for quantification of brain Hbb/Alas2 transcripts in homogenates.

SUBMITTER: Walser M

PROVIDER: S-EPMC10755301 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Brain tissue haemoglobin expression in saline-perfused vs non-perfused rodents.

Walser Marion M Karlsson Lars L Motalleb Reza R Isgaard Jörgen J Kuhn H Georg HG Åberg N David ND

Heliyon 20231207 1

Haemoglobin beta (Hbb) and delta-aminolevulinate synthase 2 (Alas2) messenger RNA (mRNA) is mainly found in immature red blood cells, reticulocytes, and not in mature erythrocytes. However, these are also expressed in other tissues such as brain cells, mostly neurons. Therefore, exact quantification of neural tissue homogenates may be confounded by remaining blood in the brain vasculature that may give falsely high values of Hbb/Alas2 expression. To investigate and compare t ...[more]

PMID: 38163098

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Dataset Information

Brain tissue haemoglobin expression in saline-perfused vs non-perfused rodents.

Publications

Brain tissue haemoglobin expression in saline-perfused vs non-perfused rodents.

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