Molecular Mechanisms of Cationic Fusogenic Liposome Interactions with Bacterial Envelopes.

ABSTRACT: Although fusogenic liposomes offer a promising approach for the delivery of antibiotic payloads across the cell envelope of Gram-negative bacteria, there is still a limited understanding of the individual nanocarrier interactions with the bacterial target. Using super-resolution microscopy, we characterize the interaction dynamics of positively charged fusogenic liposomes with Gram-negative (Escherichia coli) and Gram-positive (Bacillus subtilis) bacteria. The liposomes merge with the outer membrane (OM) of Gram-negative bacteria, while attachment or lipid internalization is observed in Gram-positive cells. Employing total internal reflection fluorescence microscopy, we demonstrated liposome fusion with model supported lipid bilayers. For whole E. coli cells, however,

SUBMITTER: Scheeder A 

PROVIDER: S-EPMC10755748 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature