Selective Single-Molecule Nanopore Detection of mpox A29 Protein Directly in Biofluids.

ABSTRACT: Single-molecule antigen detection using nanopores offers a promising alternative for accurate virus testing to contain their transmission. However, the selective and efficient identification of small viral proteins directly in human biofluids remains a challenge. Here, we report a nanopore sensing strategy based on a customized DNA molecular probe that combines an aptamer and an antibody to enhance the single-molecule detection of mpox virus (MPXV) A29 protein, a small protein with an M.W. of ca. 14 kDa. The formation of the aptamer-target-antibody sandwich structures enables efficient identification of targets when translocating through the nanopore. This technique can accurately detect A29 protein with a limit of detection of ∼11 fM and can distinguish the MPXV A29 from vaccinia virus A2

SUBMITTER: Cai S 

PROVIDER: S-EPMC10755749 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature