Heterologous Naringenin Production in the Filamentous Fungus Penicillium rubens.

ABSTRACT: Naringenin is a natural product with several reported bioactivities and is the key intermediate for the entire class of plant flavonoids. The translation of flavonoids into modern medicine as pure compounds is often hampered by their low abundance in nature and their difficult chemical synthesis. Here, we investigated the possibility to use the filamentous fungus Penicillium rubens as a host for flavonoid production. P. rubens is a well-characterized, highly engineered, traditional "workhorse" for the production of β-lactam antibiotics. We integrated two plant genes encoding enzymes in the naringenin biosynthesis pathway into the genome of the secondary metabolite-deficient P. rubens 4xKO strain. After optimization of the fermentation conditions, we obtained an excelle

SUBMITTER: Peng B 

PROVIDER: S-EPMC10755750 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature