Modulation of redox reactivity of resazurin through host-guest complexation with Cucurbit[n]uril (n = 7, 8).

ABSTRACT: Resazurin (Alamar Blue, RZ) is a widely utilized fluorescent probe for biological sensing, whose fluorescent intensity can be modulated by changing its redox states; thereby, electrochemical reactivity of RZ is of significance when designing a sensing assay. Herein, we report novel two-way electrochemical reactivity modulation of RZ using host-guest complexation with rigid molecular containers cucurbit[n]uril (CBn, n = 7, 8). The complexation between CBn and RZ is confirmed by ¹H NMR measurements and supported by computational simulation, and the binding constants are determined via UV-vis titration. Notably, the voltametric data highlights that the redox reactivity of RZ can be activated or deactivated upon encapsulation by CB8 or CB7, respectively.

SUBMITTER: Liu J 

PROVIDER: S-EPMC10755866 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature