Genome-wide detection of Wolbachia in natural Aedes aegypti populations using ddRAD-Seq.

ABSTRACT:

Background

Wolbachia, an endosymbiotic bacterium, is globally used to control arboviruses because of its ability to block arboviral replication and manipulate the reproduction of Wolbachia host, Aedes aegypti. Polymerase chain reaction (PCR)-based Wolbachia detection has been recently reported from natural Ae. aegypti populations. However, due to the technical limitations of PCR, such as primer incompatibility, PCR-based assays are not sufficiently reliable or accurate. In this study, we examined double digestion restriction site-associated DNA sequencing (ddRAD-Seq) efficiency and limitations in Wolbachia detection and quantification in field-collected Ae. aegypti natural populations in Metro Manila, the Philippines, compared with P

SUBMITTER: Muharromah AF 

PROVIDER: S-EPMC10755911 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature