Depletion of DNTTIP2 Induces Cell Cycle Arrest in Pancreatic Cancer Cells.

ABSTRACT:

Background/aim

Pancreatic cancer is one of the most lethal malignant cancers worldwide and the seventh most common cause of cancer-related death in both sexes. Herein, we analyzed open access data and discovered that expression of a gene called deoxynucleotidyltransferase terminal-interacting protein 2 (DNTTIP2) is linked to prognosis of pancreatic ductal adenocarcinoma (PDAC). We then elucidated the role of DNTTIP2 in the proliferation of pancreatic cancer cells in vitro.

Materials and methods

A WST-8 assay, cell cycle analysis, Annexin-V staining, quantitative reverse transcription-PCR, and western blot analysis were conducted to assess cell proliferation, cell cycle, apoptosis, and expression of DNTTIP2 mRNA and protein, respectively, in DNTTIP2-depleteted MIA-PaCa-2 and

SUBMITTER: Yoshizawa M 

PROVIDER: S-EPMC10756344 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature