Genetic Characterization of Pediatric Mixed Phenotype Acute Leukemia (MPAL).

ABSTRACT:

Background/aim

Mixed phenotype acute leukemia (MPAL) is a rare hematologic malignancy in which the leukemic cells cannot be assigned to any specific lineage. The lack of well-defined, pathogenetically relevant diagnostic criteria makes the clinical handling of MPAL patients challenging. We herein report the genetic findings in bone marrow cells from two pediatric MPAL patients.

Patients and methods

Bone marrow cells were examined using G-banding, array comparative genomic hybridization, RNA sequencing, reverse transcription polymerase chain reaction, Sanger sequencing, and fluorescence in situ hybridization.

Results

In the first patient, the genetic analyses revealed structural aberrations of chromosomal bands 8p11, 10p11, 11q21, and 17p11, the chimeras MLLT10::PICALM

SUBMITTER: Panagopoulos I 

PROVIDER: S-EPMC10756350 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature