Molecular cartography uncovers evolutionary and microenvironmental dynamics in sporadic colorectal tumors.

ABSTRACT: Colorectal cancer exhibits dynamic cellular and genetic heterogeneity during progression from precursor lesions toward malignancy. Analysis of spatial multi-omic data from 31 human colorectal specimens enabled phylogeographic mapping of tumor evolution that revealed individualized progression trajectories and accompanying microenvironmental and clonal alterations. Phylogeographic mapping ordered genetic events, classified tumors by their evolutionary dynamics, and placed clonal regions along global pseudotemporal progression trajectories encompassing the chromosomal instability (CIN+) and hypermutated (HM) pathways. Integrated single-cell and spatial transcriptomic data revealed recurring epithelial programs and infiltrating immune states along progression pseudotime. We discovered an immu

SUBMITTER: Heiser CN 

PROVIDER: S-EPMC10756562 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature