A potential osteogenic role for microRNA-181a-5p during palatogenesis.

ABSTRACT:

Background

In a previous study, we found that the highly conserved hsa-miR-181a-5p is downregulated in palatal fibroblasts of non-syndromic cleft palate-only infants.

Objectives

To analyze the spatiotemporal expression pattern of mmu-miR-181a-5p during palatogenesis and identify possible mRNA targets and their involved molecular pathways.

Material and methods

The expression of mmu-miR-181a-5p was analyzed in the developing palates of mouse embryos from E11 to E18 using qPCR and ISH. Mouse embryonic palatal mesenchyme cells from E13 were used to analyze mmu-miR-181a-5p expression during osteogenic differentiation. Differential mRNA expression and target identification were analyzed using whole transcriptome RNA sequencing after transfection with a mmu-miR-181a-5p mimic

SUBMITTER: Schoen C 

PROVIDER: S-EPMC10756689 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature