Differential Expression Profile of Salivary oncomiRNAs among Smokeless Tobacco Users.

ABSTRACT:

Objective

 The aim of this study was to evaluate the expression of selected salivary oncomiRNAs among smokeless tobacco users and nonsmokers.

Materials and methods

 Twenty-five subjects with chronic smokeless tobacco habit (> 1 year) and 25 nonsmokers were selected for this study. MicroRNA was extracted from saliva samples using the miRNeasy Kit (Qiagen, Hilden, Germany). The forward primers used in the reactions include hsa-miR-21-5p, hsa-miR-146a-3p, hsa-miR-155-3p, and hsa-miR-199a-3p. Relative expression of miRNAs was calculated using the 2-ΔΔCt method. Fold change is calculated by raising 2 to the power of the negative ΔΔCT value.

Statistical analysis

 Statistical analysis was carried out using GraphPad Prism 5 software. A p-value less than 0.05 was consid

SUBMITTER: Al-Rawi NH 

PROVIDER: S-EPMC10756836 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature