Effect of low frequency ultrasound waves on the morphology and viability of cultured human gingival fibroblasts.

ABSTRACT:

Objectives

The aim of this study was to investigate the effect of the vibration amplitude of mechanical ultrasound waves (27 kHz) on the viability and morphology of human gingival fibroblasts (hGFs) when cultured on a biomaterial substrate.

Method

hGFs were seeded on tissue culture plates (TCPs) and an Ti6Al4V titanium alloy surface in two groups for three days and seven days of cell culture. The cells were subjected to three vibration amplitudes for 20 min each day. Scanning electron microscope (SEM) images were used to characterize cell morphology.

Results

Experiments showed that hGF cells became detached from their plates at a vibration amplitude comparable to an intensity of 260 mW/cm2. In addition, hGfs that received a vibrational amplitude comparable to an inten

SUBMITTER: Afshari M 

PROVIDER: S-EPMC10757313 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature