Isolation and molecular characteristics of a recombinant feline calicivirus from Qingdao, China.

ABSTRACT: Feline calicivirus (FCV) is a highly contagious pathogen seriously affecting the upper respiratory tract and producing oral diseases in the feline. Despite widespread vaccination, the prevalence of FCV remains high. In this study, the FCV qingdao (qd)/2019/china was isolated from a domestic feline oropharyngeal swab collected from Qingdao, China. The virus was purified using the plaque assay and identified using the Polymerase chain reaction and indirect immunofluorescence assay methods, the capsid amino acid, VP1 of qd/2019/china, showed sequence identity with the other isolates ranging between 83.90% (ym3/2001/jp) and 91.10% (CH-JL4). The sequence of the capsid amino acid revealed qd/2019/china to be closely related to CH-JL4 and clustered with CH-JL4 in the phylogenetic tree. The

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10758007 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature