Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization.

ABSTRACT: Rationale: Cancer local recurrence increases the mortality of patients, and might be caused by field cancerization, a pre-malignant alteration of normal epithelial cells. It has been suggested that cancer-derived small extracellular vesicles (CDEs) may contribute to field cancerization, but the underlying mechanisms remain poorly understood. In this study, we aim to identify the key regulatory factors within recipient cells under the instigation of CDEs. Methods: In vitro experiments were performed to demonstrate that CDEs promote the expression of CREPT in normal epithelial cells. TMT-based quantitative mass spectrometry was employed to investigate the proteomic differences between normal cells and tumor cells. Loss-of-function approaches by CRISPR-Cas9 system were used to a

SUBMITTER: Lin Y 

PROVIDER: S-EPMC10758052 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature