Multiple HPV integration mode in the cell lines based on long-reads sequencing.

ABSTRACT:

Background

The integration of human papillomavirus (HPV) is closely related to the occurrence of cervical cancer. However, little is known about the complete state of HPV integration into the host genome.

Methods

In this study, three HPV-positive cell lines, HeLa, SiHa, and CaSki, were subjected to NANOPORE long-read sequencing to detect HPV integration. Analysis of viral integration patterns using independently developed software (HPV-TSD) yielded multiple complete integration patterns for the three HPV cell lines.

Results

We found distinct differences between the integration patterns of HPV18 and HPV16. Furthermore, the integration characteristics of the viruses were significantly different, even though they all belonged to HPV16 integration. The HPV integration in

SUBMITTER: Cui X 

PROVIDER: S-EPMC10758443 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature