The oncogenic role and regulatory mechanism of PGK1 in human non-small cell lung cancer.

ABSTRACT:

Background

Phosphoglycerate kinase 1 (PGK1) is a metabolic enzyme that participates in various biological and pathological processes. Dysregulated PGK1 has been observed in numerous malignancies. However, whether and how PGK1 affects non-small cell lung cancer (NSCLC) is not yet fully elucidated.

Methods

Herein, the non-metabolic function of PGK1 in NSCLC was explored by integrating bioinformatics analyses, cellular experiments, and nude mouse xenograft models. The upstream regulators and downstream targets of PGK1 were examined using multiple techniques such as RNA sequencing, a dual-luciferase reporter assay, Co-immunoprecipitation, and Western blotting.

Results

We confirmed that PGK1 was upregulated in NSCLC and this upregulation was associated with poor prognosis.

SUBMITTER: Tian T 

PROVIDER: S-EPMC10759362 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature