Proteomic profiling identifies SPP1 associated with rapidly progressive interstitial lung disease in anti-MDA5-positive dermatomyositis.

ABSTRACT:

Background

Anti-melanoma differentiation-associated gene five antibody positive (MDA5⁺) dermatomyositis (DM) is significantly associated with rapidly progressive interstitial lung disease (RP-ILD). Early detection of RP-ILD remains a major challenge. This study aims to identify and validate prognostic factors for RP-ILD in MDA5⁺ DM patients.

Methods

Plasma samples from 20 MDA5⁺ DM patients and 10 healthy controls (HC) were collected for proteomic analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The proteins of interest were validated in independent samples (20 HC, 20 MDA5⁺ DM with RP-ILD, and 20 non-RP-ILD patients) with enzyme-linked immunosorbent assay (ELISA).

Results

A total of 413 diffe

SUBMITTER: Qiu Y 

PROVIDER: S-EPMC10759429 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature