NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes.

ABSTRACT:

Background

Neuroepithelial transforming gene 1 (NET1) is a RhoA subfamily guanine nucleotide exchange factor that governs a wide array of biological processes. However, its roles in meiotic oocyte remain unclear. We herein demonstrated that the NET1-HACE1-RAC1 pathway mediates meiotic defects in the progression of oocyte maturation.

Methods

NET1 was reduced using a specific small interfering RNA in mouse oocytes. Spindle assembly, chromosomal alignment, the actin cap, and chromosomal spreads were visualized by immunostaining and analyzed under confocal microscopy. We also applied mass spectroscopy, and western blot analysis for this investigation.

Results

Our results revealed that NET1 was localized to the nucleus at the GV stage, and that after GVBD, NET1 was localiz

SUBMITTER: Wang S 

PROVIDER: S-EPMC10759572 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature