Development of Oxadiazolone Activity-Based Probes Targeting FphE for Specific Detection of <i>S. aureus</i> Infections.

ABSTRACT: Staphylococcus aureus is a major human pathogen responsible for a wide range of systemic infections. Since its propensity to form biofilms in vivo poses formidable challenges for both detection and treatment, tools that can be used to specifically image S. aureus biofilms are highly valuable for clinical management. Here we describe the development of oxadiazolonebased activity-based probes to target the S. aureus-specific serine hydrolase FphE. Because this enzyme lacks homologs in other bacteria, it is an ideal target for selective imaging of S. aureus infections. Using X-ray crystallography, direct cell labeling and mouse models of infection we demonstrate that oxadiazolone-based probes enable specific labeling of S. aureus bacteria through the

SUBMITTER: Jo J 

PROVIDER: S-EPMC10760020 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature