Hybracter: Enabling Scalable, Automated, Complete and Accurate Bacterial Genome Assemblies.

ABSTRACT: Improvements in the accuracy and availability of long-read sequencing mean that complete bacterial genomes are now routinely reconstructed using hybrid (i.e. short- and long-reads) assembly approaches. Complete genomes allow a deeper understanding of bacterial evolution and genomic variation beyond single nucleotide variants (SNVs). They are also crucial for identifying plasmids, which often carry medically significant antimicrobial resistance (AMR) genes. However, small plasmids are often missed or misassembled by long-read assembly algorithms. Here, we present Hybracter which allows for the fast, automatic, and scalable recovery of near-perfect complete bacterial genomes using a long-read first assembly approach. Hybracter can be run either as a hybrid assembler or as a long-read only as

SUBMITTER: Bouras G 

PROVIDER: S-EPMC10760025 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature