A molecular toolkit for heterologous protein secretion across <i>Bacteroides</i> species.

ABSTRACT: Bacteroides species are abundant and prevalent stably colonizing members of the human gut microbiota, making them a promising chassis for developing long-term interventions for chronic diseases. Engineering these bacteria as on-site production and delivery vehicles for biologic drugs or diagnostics, however, requires efficient heterologous protein secretion tools, which are currently lacking. To address this limitation, we systematically investigated methods to enable heterologous protein secretion in Bacteroides using both endogenous and exogenous secretion systems. Here, we report a collection of secretion carriers that can export functional proteins across multiple Bacteroides species at high titers. To understand the mechanistic drivers of Bacteroides secret

SUBMITTER: Yeh YH 

PROVIDER: S-EPMC10760143 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature