Analysis of clinical <i>Candida parapsilosis</i> isolates reveals copy number variation in key fluconazole resistance genes.

ABSTRACT: We used whole-genome sequencing to analyse a collection of 35 fluconazole resistant and 7 susceptible Candida parapsilosis isolates together with coverage analysis and GWAS techniques to identify new mechanisms of fluconazole resistance. Phylogenetic analysis shows that although the collection is diverse, two probable outbreak groups were identified. We identified copy number variation of two genes, ERG11 and CDR1B, in resistant isolates. Two strains have a CNV at the ERG11 locus; the entire ORF is amplified in one, and only the promoter region is amplified in the other. We show the annotated telomeric gene CDR1B is actually an artefactual in silico fusion of two highly similar neighbouring CDR genes due to an assembly error in the C. par

SUBMITTER: Bergin S 

PROVIDER: S-EPMC10760152 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature