The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples.

ABSTRACT: In 2015, we launched the mesoSPIM initiative (www.mesospim.org), an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of light-sheet microscopy. Here, we introduce the next-generation mesoSPIM ("Benchtop") with significantly increased field of view, improved resolution, higher throughput, more affordable cost and simpler assembly compared to the original version. We developed a new method for testing objectives, enabling us to select detection objectives optimal for light-sheet imaging with large-sensor sCMOS cameras. The new mesoSPIM achieves high spatial resolution (1.5 μm laterally, 3.3 μm axially) a

SUBMITTER: Vladimirov N 

PROVIDER: S-EPMC10760166 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature