Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice.

ABSTRACT: Mammalian meiotic recombination proceeds via repair of hundreds of programmed DNA double-strand breaks, which requires choreographed binding of RPA, DMC1, and RAD51 to single-stranded DNA substrates. High-resolution in vivo binding maps of these proteins provide insights into the underlying molecular mechanisms. When assayed in F₁-hybrid mice, these maps can distinguish the broken chromosome from the chromosome used as template for repair, revealing more mechanistic detail and enabling the structure of the recombination intermediates to be inferred. By applying CRISPR-Cas9 mutagenesis directly on F₁-hybrid embryos, we have extended this approach to explore the molecular detail of recombination when a key component is knocked out. As a proof of concept, we have generat

SUBMITTER: Davies B 

PROVIDER: S-EPMC10760447 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature