Cohesin and CTCF do not assemble TADs in <i>Xenopus</i> sperm and male pronuclei.

ABSTRACT: Paternal genomes are compacted during spermiogenesis and decompacted following fertilization. These processes are fundamental for inheritance but incompletely understood. We analyzed these processes in the frog Xenopus laevis, whose sperm can be assembled into functional pronuclei in egg extracts in vitro. In such extracts, cohesin extrudes DNA into loops, but in vivo cohesin only assembles topologically associating domains (TADs) at the mid-blastula transition (MBT). Why cohesin assembles TADs only at this stage is unknown. We first analyzed genome architecture in frog sperm and compared it to human and mouse. Our results indicate that sperm genome organization is conserved between frogs and humans and occurs without formation of TADs. TADs can be detected in mouse sperm samples, a

SUBMITTER: Jessberger G 

PROVIDER: S-EPMC10760524 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature