A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture.

ABSTRACT: Self-cleaving ribozymes are versatile tools for synthetic biologists when it comes to controlling gene expression. Up to date, 12 different classes are known, and over the past decades more and more details about their structure, cleavage mechanisms and natural environments have been uncovered. However, when these motifs are applied to mammalian gene expression constructs, the outcome can often be unexpected. A variety of factors, such as surrounding sequences and positioning of the ribozyme influences the activity and hence performance of catalytic RNAs. While some information about the efficiency of individual ribozymes (each tested in specific contexts) is known, general trends obtained from standardized, comparable experiments are lacking, complicating decisions such as which ribozyme

SUBMITTER: Klage D 

PROVIDER: S-EPMC10761166 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature