Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function.

ABSTRACT: Spleen marginal zone (MZ) B cells are important for antibody responses against blood-borne antigens. The signals they use to detect exposure to blood are not well defined. Here, using intravital two-photon microscopy in mice, we observe transient contacts between MZ B cells and red blood cells that are in flow. We show that MZ B cells use adhesion G-protein-coupled receptor ADGRE5 (CD97) for retention in the spleen. CD97 function in MZ B cells depends on its ability to undergo autoproteolytic cleavage and signaling via Gα₁₃ and ARHGEF1. Red blood cell expression of the CD97 ligand CD55 is required for MZ B cell homeostasis. Applying a pulling force on CD97-transfected cells using an optical C-trap and CD55⁺ beads leads to accumulation of active RhoA and membrane retra

SUBMITTER: Liu D 

PROVIDER: S-EPMC10761324 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature