Screening and validation of double allele-specific binding F-primers for the measurement of antihypertensive pharmacogenomics.

ABSTRACT:

Objective

Previous studies have proposed that genetic polymorphisms of CYP2D6^*10, ADRB1, NPPA, CYP3A5^*3, ACE, CYP2C9^*3, and AGTR1 are involved in antihypertensive pharmacogenomics. The purpose of this study is to develop an amplification analysis using double allele-specific (AS) binding primers for accurate measurement of antihypertensive pharmacogenomics.

Methods

To establish a quadruplex quantitative PCR (qPCR) analysis for genotyping of CYP2D6^*10, ADRB1 (1165 G>C), NPPA (2238 T>C) and CYP3A5^*3, and a triplex qPCR analysis for genotyping of ACE (I/D), CYP2C9^*3 and AGTR1 (1166 A>C), mismatch AS F-primers were screened by detection of plasmid/gDNA, and were validated by agreement analysis/reproducibility eval

SUBMITTER: Ping Y 

PROVIDER: S-EPMC10761462 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature