Gene architecture is a determinant of the transcriptional response to bulky DNA damages.

ABSTRACT: Bulky DNA damages block transcription and compromise genome integrity and function. The cellular response to these damages includes global transcription shutdown. Still, active transcription is necessary for transcription-coupled repair and for induction of damage-response genes. To uncover common features of a general bulky DNA damage response, and to identify response-related transcripts that are expressed despite damage, we performed a systematic RNA-seq study comparing the transcriptional response to three independent damage-inducing agents: UV, the chemotherapy cisplatin, and benzo[a]pyrene, a component of cigarette smoke. Reduction in gene expression after damage was associated with higher damage rates, longer gene length, and low GC content. We identified genes with relatively highe

SUBMITTER: Merav M 

PROVIDER: S-EPMC10761554 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature