Dual-wavelength metalens enables Epi-fluorescence detection from single molecules.

ABSTRACT: Single molecule fluorescence spectroscopy is at the heart of molecular biophysics research and the most sensitive biosensing assays. The growing demand for precision medicine and environmental monitoring requires the creation of miniaturized and portable sensing platforms. However, the need for highly sophisticated objective lenses has precluded the development of single molecule detection systems for truly portable devices. Here, we propose a dielectric metalens device of submicrometer thickness to excite and collect light from fluorescent molecules instead of an objective lens. The high numerical aperture, high focusing efficiency, and dual-wavelength operation of the metalens enable the implementation of fluorescence correlation spectroscopy with a single Alexa 647 molecule in the focal

SUBMITTER: Barulin A 

PROVIDER: S-EPMC10761847 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature