Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection.

ABSTRACT: Host anti-viral factors are essential for controlling SARS-CoV-2 infection but remain largely unknown due to the biases of previous large-scale studies toward pro-viral host factors. To fill in this knowledge gap, we perform a genome-wide CRISPR dropout screen and integrate analyses of the multi-omics data of the CRISPR screen, genome-wide association studies, single-cell RNA-Seq, and host-virus proteins or protein/RNA interactome. This study uncovers many host factors that are currently underappreciated, including the components of V-ATPases, ESCRT, and N-glycosylation pathways that modulate viral entry and/or replication. The cohesin complex is also identified as an anti-viral pathway, suggesting an important role of three-dimensional chromatin organization in mediating host-viral intera

SUBMITTER: Hou J 

PROVIDER: S-EPMC10761986 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature