Leucine aminopeptidase1 controls egg deposition and hatchability in male Aedes aegypti mosquitoes.

ABSTRACT: Aedes aegypti are vectors for several arboviruses infecting hundreds of millions of people annually. Controlling mosquito populations by regulating their reproduction is a potential strategy to minimize viral transmission in the absence of effective antiviral therapies or vaccines. Here, we demonstrate that leucine aminopeptidase1 (LAP1), detected by a SWATH-MS-based proteomic screen of female spermathecae, is a crucial determinant in mosquito population expansion. Mitochondrial defects and aberrant autophagy of sperm in LAP1 mutant males (LAP1^-/-), prepared using CRISPR/Cas9 system, result in a reduction of reproduction in wild-type females that mated with them. The fitness of LAP1^-/- males is strong enough to efficiently transmit genetic changes to mosquito populati

SUBMITTER: Sun X 

PROVIDER: S-EPMC10762072 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature