Mapping nucleosome-resolution chromatin organization and enhancer-promoter loops in plants using Micro-C-XL.

ABSTRACT: Although chromatin organizations in plants have been dissected at the scales of compartments and topologically associating domain (TAD)-like domains, there remains a gap in resolving fine-scale structures. Here, we use Micro-C-XL, a high-throughput chromosome conformation capture (Hi-C)-based technology that involves micrococcal nuclease (instead of restriction enzymes) and long cross-linkers, to dissect single nucleosome-resolution chromatin organization in Arabidopsis. Insulation analysis reveals more than 14,000 boundaries, which mostly include chromatin accessibility, epigenetic modifications, and transcription factors. Micro-C-XL reveals associations between RNA Pols and local chromatin organizations, suggesting that gene transcription substantially contributes to the establishment of

SUBMITTER: Sun L 

PROVIDER: S-EPMC10762229 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature