Lipid Enriched Reduced Nutrient Culture Medium Improves Bovine Blastocyst Formation.

ABSTRACT: The refinement of embryo culture media is essential in improving embryo viability and in vitro production efficiency. Our previous work demonstrated that the nutrients (carbohydrates, amino acids, and vitamins) in traditional culture media far exceed the need for an embryo and producing developmentally competent embryos in a reduced nutrient environment is feasible. Here, we aim to evaluate the impact of exogenous lipid and L-carnitine supplementation on bovine blastocyst development and refine our RN condition further. Zygotes were cultured in the control medium (100% nutrients) and reduced nutrient media containing 6.25% of the standard nutrient concentrations supplemented with L-carnitine and lipid free or lipid rich BSA. Increased blastocyst development was observed in the reduced nutr

SUBMITTER: Pasquariello R 

PROVIDER: S-EPMC10762584 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature