Characterization of circulating extracellular traps and immune responses to citrullinated LL37 in psoriasis.

ABSTRACT:

Background

The DNA-binding peptide LL37 is a suspected autoantigen in psoriasis. It can be found in neutrophil extracellular traps (NETs) which have been suggested to play a role in the pathogenesis of the disease. Citrullination, the conversion of peptidyl-arginine into peptidyl-citrulline, can be implicated in the formation of NETs. We hypothesized that citrullination increases LL37 immunogenicity and that NETs are a source of LL37.

Objectives

We aimed to characterize cytokine responses of B cells and T cells to native and citrullinated LL37 (citLL37) and determine the prevalence and composition of circulating NETs in patients with psoriasis and healthy blood donors (HDs).

Methods

Mononuclear cells (MNCs) and serum were isolated from 20 HDs and 20 patients with psor

SUBMITTER: Martin Monreal MT 

PROVIDER: S-EPMC10762777 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature