MicroRNA-622 upregulates cell cycle process by targeting FOLR2 to promote CRC proliferation.

ABSTRACT:

Background

Epigenetic alterations contribute greatly to the development and progression of colorectal cancer, and effect of aberrant miR-622 expression is still controversial. This study aimed to discover miR-622 regulation in CRC proliferation.

Methods

miR-622 expression and prognosis were analyzed in clinical CRC samples from Nanfang Hospital. miR-622 regulation on cell cycle and tumor proliferation was discovered, and FOLR2 was screened as functional target of miR-622 using bioinformatics analysis, which was validated via dual luciferase assay and gain-of-function and loss-of-function experiments both in vitro and in vivo.

Results

miR-622 overexpression in CRC indicated unfavorable prognosis and it regulated cell cycle to promote tumor growth both in vitro and in v

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10763126 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature