CD317⁺ MSCs expanded with chemically defined media have enhanced immunological anti-inflammatory activities.

ABSTRACT:

Background

Although both preclinical and clinical studies have shown the great application potential of MSCs (mesenchymal stem/stromal cells) in treating many kinds of diseases, therapeutic inconsistency resulting from cell heterogeneity is the major stumbling block to their clinical applications. Cell population diversity and batch variation in the cell expansion medium are two major inducers of MSC heterogeneity.

Methods

Cell population diversity was investigated through single-cell RNA sequencing analysis of human MSCs derived from the umbilical cord and expanded with fully chemically defined medium in the current study. Then, the MSC subpopulation with enhanced anti-inflammatory effects was studied in vitro and in vivo.

Results

Our data showed that MSCs contain di

SUBMITTER: Song J 

PROVIDER: S-EPMC10763464 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature