Characterization of gastric cancer-stimulated signaling pathways and function of CTGF in cancer-associated fibroblasts.

ABSTRACT:

Background

Cancer-associated fibroblasts (CAFs) are key components of the tumor microenvironment (TME) that play an important role in cancer progression. Although the mechanism by which CAFs promote tumorigenesis has been well investigated, the underlying mechanism of CAFs activation by neighboring cancer cells remains elusive. In this study, we aim to investigate the signaling pathways involved in CAFs activation by gastric cancer cells (GC) and to provide insights into the therapeutic targeting of CAFs for overcoming GC.

Methods

Alteration of receptor tyrosine kinase (RTK) activity in CAFs was analyzed using phospho-RTK array. The expression of CAFs effector genes was determined by RT-qPCR or ELISA. The migration and invasion of GC cells co-cultured with CAFs were examined

SUBMITTER: Choi KM 

PROVIDER: S-EPMC10763493 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature