Ligand Binding-Induced Cellular Membrane Deformation is Correlated with the Changes in Membrane Stiffness.

ABSTRACT: Study interaction between ligands and protein receptors is a key step for biomarker research and drug discovery. In situ measurement of cell surface membrane protein binding on whole cells eliminates the cost and pitfalls associated with membrane protein purification. Ligand binding to membrane protein was recently found to induce nanometer-scale cell membrane deformations, which can be monitored with real-time optical imaging to quantify ligand/protein binding kinetics. However, the insight into this phenomenon has still not been fully understood. We hypothesize that ligand binding can change membrane stiffness, which induces membrane deformation. To investigate this, cell height and membrane stiffness changes upon ligand binding are measured using atomic force microscopy (AFM). Wheat ger

SUBMITTER: Kolay J 

PROVIDER: S-EPMC10763494 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature