Characterization of the RofA regulon in the pandemic M1_global and emergent M1_UK lineages of Streptococcus pyogenes.

ABSTRACT: The standalone regulator RofA is a positive regulator of the pilus locus in Streptococcus pyogenes. Found in only certain emm genotypes, RofA has been reported to regulate other virulence factors, although its role in the globally dominant emm1 S. pyogenes is unclear. Given the recent emergence of a new emm1 (M1_UK) toxigenic lineage that is distinguished by three non-synonymous SNPs in rofA, we characterized the rofA regulon in six emm1 strains that are representative of the two contemporary major emm1 lineages (M1_global and M1_UK) using RNAseq analysis, and then determined the specific role of the M1_UK-specific rofA SNPs. Deletion of rofA in three M1_global str

SUBMITTER: Zhi X 

PROVIDER: S-EPMC10763501 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature