A hsa_circ_001726 axis regulated by E2F6 contributes to metastasis of hepatocellular carcinoma.

ABSTRACT:

Background

CircRNAs participate in the development of hepatocellular carcinoma (HCC). This work aims to explore the key tumor promoting circRNA as a gene therapy target.

Methods

The differentially expressed gene circRNAs in HCC tumor tissues was identified by mining GSE121714 dataset. EdU staining, wound healing, transwell invasion assay, TUNEL staining and western blotting examined proliferation, migration, invasion, apoptosis and epithelial mesenchymal transition (EMT). Xenograft mouse model and orthotopic transplantation tumor mouse model were constructed to verify the role of hsa_circ_001726 in growth and metastasis of HCC. The relationship among CCT2, E2F6, hsa_circ_001726, miR-671-5p and PRMT9 was identified by RNA-fluorescence in situ hybridization, luciferase reporte

SUBMITTER: Ai J 

PROVIDER: S-EPMC10763683 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature