Slide-tags enables single-nucleus barcoding for multimodal spatial genomics.

ABSTRACT: Recent technological innovations have enabled the high-throughput quantification of gene expression and epigenetic regulation within individual cells, transforming our understanding of how complex tissues are constructed^1-6. However, missing from these measurements is the ability to routinely and easily spatially localize these profiled cells. We developed a strategy, Slide-tags, in which single nuclei within an intact tissue section are tagged with spatial barcode oligonucleotides derived from DNA-barcoded beads with known positions. These tagged nuclei can then be used as an input into a wide variety of single-nucleus profiling assays. Application of Slide-tags to the mouse hippocampus positioned nuclei at less than 10 μm spatial resolution and delivered whole-transcriptome da

SUBMITTER: Russell AJC 

PROVIDER: S-EPMC10764288 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature