Passaging of gingival fibroblasts from periodontally healthy and diseased sites upregulates osteogenesis-related genes.

ABSTRACT: To investigate biological processes of the periodontium, in vitro primary cell models have been established. To study the biology of the gingiva, primary gingival fibroblast cell models are widely used. For such experiments, cells need to be expanded and passaged. A key assumption is that primary cells maintain most of their original characteristics they have in situ. The aim of this research is to explore the impact of early passaging on selected gene expression of human gingival fibroblast cells. For this purpose, gene expression from the outgrowth of the resected tissues until the fourth passage was followed for nine tissue samples, from both healthy and diseased sites. Micrographs were taken from the cultures, RNA was extracted from the samples of each passage and quantitative PCR was

SUBMITTER: Karlis GD 

PROVIDER: S-EPMC10764533 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature